Training & Documents
From BioImagingUKWiki
Contents |
Sample preparation
Light Microscopy Documents
- Localisation Microscopy (STORM/PALM) Coverglass Guide (745 kB)
- Localisation Microscopy (STORM/GSDIM) Immunolabelling Guide (532 kB)
Light Microscopy Movies
Please upload sample preparation movies here
Electron Microscopy Documents
Please upload sample preparation protocols here
3View (Serial Block Face SEM)
Electron Microscopy Movies
Please upload sample preparation movies here
Microscope usage
Light Microscopy Documents
Please upload microscope usage protocols here
Confocal microscopes
- Leica SP2 Upright Quickstart Guide (141 kB)
- Leica SP5 Upright Quickstart Guide (233 kB)
- Leica SP5 Inverted Quickstart Guide (217 kB)
- Leica SP5 with Resonant Scanner Quickstart Guide (217 kB)
- Zeiss LSM 510 Quickstart Guide (353 kB)
Widefield microscopes
- Zeiss Axiovert 200M with Hamamatsu Volocity Software Quickstart Guide (281 kB)
- Zeiss Axiovert 200M with Simple PCI Software Quickstart Guide (400 kB)
Light Microscopy Movies
Please upload microscope usage movies here
Electron Microscopy Documents
Please upload microscope usage protocols here
3View (Serial Block Face SEM)
Electron Microscopy Movies
Please upload microscope usage movies here
Microscope maintenance
Light Microscopy Documents
Please upload microscope maintenance protocols here
Light Microscopy Movies
- Home built PALM/STORM microscope
- 3D PALM/STORM with astigmatism
- Custom laser control
- Maintenance videos including Cleaning Microscope Optics & Bulb Replacement
- Finding dirt in your optical system
Electron Microscopy Documents
Please upload microscope maintenance protocols here
3View (Serial Block Face SEM)
Electron Microscopy Movies
Please upload microscope maintenance movies here
General microscopy
Light Microscopy Documents
- Good Imaging Practice Overview (343 kB)
- Comparison of SIM and STORM results (963 kB)
Light Microscopy Movies
Please upload general microscopy movies here
- Eyepieces
- Objectives and eyepieces
- Features of an objective lens
- Phase, Polarization & DIC Microscopy
- Dark Field, Phase Contrast, Polarisation and DIC
- Polarized Light and its Interaction with Material
- Correcting for spherical aberration
- Demystifying microscopes - disassembling a Nikon Ti Eclipse
- Lenses and image formation
- What you can learn with a light microscope
- Choosing the right microscopy technique
- Designing a fluorescence microscopy experiment
- Introduction to Fluorescence Microscopy
- Deconvolution
- Resolution in microscopy
Light Microscopy Advanced Techniques & Methods
- Fourier Space (Image formation)
- Hight throughput microscopy
- Deconvolution
- Optogenetics
- Optical sectioning and confocal microscopy
- TIRF
- Two photon microscopy
F Words
- FRET
- Imaging protein-protein interactions in-vivo (FRET)
- Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
- FLIM
- Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging
- Lateral Diffusion and Exocytosis of Membrane Proteins in Cultured Neurons Assessed using Fluorescence Recovery and Fluorescence-loss Photobleaching
- FRAP and Photoactivation
Super-Resolution Microscopy
- Super-Resolution Microscopy
- Super-resolution microscopy: STED
- Super-Resolution Microscopy: Localisation Microscopy
- Super-Resolution Microscopy: SIM
- Test Samples for Optimizing STORM Super-Resolution Microscopy
Light Sheet / SPIM
Electron Microscopy Documents
Please upload general microscopy protocols here
3View (Serial Block Face SEM)
Electron Microscopy Movies
Please upload general microscopy movies here
Software
Documents
- Icy: an open community platform for bioimage informatics
- QuickPALM software for localisation microscopy
- rainSTORM software for localisation microscopy
- rainSTORM user guide (1.31 MB)
