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Revision as of 20:00, 8 January 2015 (view source) Lcollinson (Talk | contribs) ← Older edit		Revision as of 11:01, 31 March 2015 (view source) Lcollinson (Talk | contribs) (→Electron Microscopy Documents) Newer edit →
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	== Electron Microscopy Documents ==		== Electron Microscopy Documents ==
	Please upload sample preparation protocols here		Please upload sample preparation protocols here
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		+	'''3View (Serial Block Face SEM)'''
	== Electron Microscopy Movies ==		== Electron Microscopy Movies ==

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Revision as of 11:01, 31 March 2015

Contents 1 Sample preparation 1.1 Light Microscopy Documents 1.2 Light Microscopy Movies 1.3 Electron Microscopy Documents 1.4 Electron Microscopy Movies 2 Microscope usage 2.1 Light Microscopy Documents 2.2 Light Microscopy Movies 2.3 Electron Microscopy Documents 2.4 Electron Microscopy Movies 3 Microscopy maintenance 3.1 Light Microscopy Documents 3.2 Light Microscopy Movies 3.3 Electron Microscopy Documents 3.4 Electron Microscopy Movies 4 General microscopy 4.1 Light Microscopy Documents 4.2 Light Microscopy Movies 4.3 Light Microscopy Advanced Techniques & Methods 4.4 Electron Microscopy Documents 4.5 Electron Microscopy Movies 5 Software 5.1 Documents 5.2 Movies

Sample preparation

Light Microscopy Documents

• Localisation Microscopy (STORM/PALM) Coverglass Guide (745 kB)
• Localisation Microscopy (STORM/GSDIM) Immunolabelling Guide (532 kB)

Light Microscopy Movies

Please upload sample preparation movies here

• Test Samples for Optimizing STORM Super-Resolution Microscopy
• Fluorescent proteins

Electron Microscopy Documents

Please upload sample preparation protocols here

3View (Serial Block Face SEM)

Electron Microscopy Movies

Please upload sample preparation movies here

Microscope usage

Light Microscopy Documents

Please upload microscope usage protocols here

Confocal microscopes

• Leica SP2 Upright Quickstart Guide (141 kB)
• Leica SP5 Upright Quickstart Guide (233 kB)
• Leica SP5 Inverted Quickstart Guide (217 kB)
• Leica SP5 with Resonant Scanner Quickstart Guide (217 kB)
• Zeiss LSM 510 Quickstart Guide (353 kB)

Widefield microscopes

• Zeiss Axiovert 200M with Hamamatsu Volocity Software Quickstart Guide (281 kB)
• Zeiss Axiovert 200M with Simple PCI Software Quickstart Guide (400 kB)

Light Microscopy Movies

Please upload microscope usage movies here

• Ti Live Cell System Setup

Electron Microscopy Documents

Please upload microscope usage protocols here

Electron Microscopy Movies

Please upload microscope usage movies here

Microscopy maintenance

Light Microscopy Documents

Please upload microscope maintenance protocols here

Light Microscopy Movies

• Home built PALM/STORM microscope
• 3D PALM/STORM with astigmatism
• Custom laser control
• Maintenance videos including Cleaning Microscope Optics & Bulb Replacement
• Finding dirt in your optical system

Electron Microscopy Documents

Please upload microscope maintenance protocols here

Electron Microscopy Movies

Please upload microscope maintenance movies here

General microscopy

Light Microscopy Documents

• Good Imaging Practice Overview (343 kB)
• Comparison of SIM and STORM results (963 kB)

Light Microscopy Movies

Please upload general microscopy movies here

• Eyepieces
• Objectives and eyepieces
• Features of an objective lens
• Phase, Polarization & DIC Microscopy
• Dark Field, Phase Contrast, Polarisation and DIC
• Polarized Light and its Interaction with Material
• Correcting for spherical aberration
• Demystifying microscopes - disassembling a Nikon Ti Eclipse

• Lenses and image formation
• What you can learn with a light microscope
• Choosing the right microscopy technique
• Designing a fluorescence microscopy experiment
• Introduction to Fluorescence Microscopy
• Deconvolution
• Resolution in microscopy

Light Microscopy Advanced Techniques & Methods

• Fourier Space (Image formation)
• Hight throughput microscopy
• Deconvolution
• Optogenetics
• Optical sectioning and confocal microscopy
• TIRF
• Two photon microscopy

F Words

• FRET
• Imaging protein-protein interactions in-vivo (FRET)
• Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
• FLIM
• Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging
• Lateral Diffusion and Exocytosis of Membrane Proteins in Cultured Neurons Assessed using Fluorescence Recovery and Fluorescence-loss Photobleaching
• FRAP and Photoactivation

Super-Resolution Microscopy

• Super-Resolution Microscopy
• Super-resolution microscopy: STED
• Super-Resolution Microscopy: Localisation Microscopy
• Super-Resolution Microscopy: SIM
• Test Samples for Optimizing STORM Super-Resolution Microscopy

Light Sheet / SPIM

• OpenSPIM
• Dual view SPIM (DISPIM)
• Light Sheet/SPIM

Electron Microscopy Documents

Please upload general microscopy protocols here

Electron Microscopy Movies

Please upload general microscopy movies here

Software

Documents

• Icy: an open community platform for bioimage informatics
• QuickPALM software for localisation microscopy
• rainSTORM software for localisation microscopy
• rainSTORM user guide (1.31 MB)

Movies

• PALM3D
• Installation and Basics of ImageJ
• RGB and grayscale montage in ImageJ
• ImageJ Seminar
• Automated Cell Counting in ImageJ