Training & Documents
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- | + | = Sample preparation = | |
- | == | + | == Light Microscopy Documents == |
+ | |||
+ | *[http://www.bioimaginguk.org/images/a/a3/Glass.pdf Localisation Microscopy (STORM/PALM) Coverglass Guide] (745 kB) | ||
+ | *[http://www.bioimaginguk.org/images/5/5c/Localisation_Microscopy_Immunolabelling.pdf Localisation Microscopy (STORM/GSDIM) Immunolabelling Guide] (532 kB) | ||
+ | |||
+ | == Light Microscopy Movies == | ||
+ | Please upload sample preparation movies here | ||
+ | |||
+ | *[http://www.jove.com/video/50579/test-samples-for-optimizing-storm-super-resolution-microscopy Test Samples for Optimizing STORM Super-Resolution Microscopy] | ||
+ | *[http://www.ibiology.org/ibioeducation/taking-courses/ibiology-microscopy-short-course/fluorescent-proteins-2.html Fluorescent proteins] | ||
+ | |||
+ | == Electron Microscopy Documents == | ||
Please upload sample preparation protocols here | Please upload sample preparation protocols here | ||
- | + | '''3View (Serial Block Face SEM)''' | |
- | + | ||
- | == | + | == Electron Microscopy Movies == |
Please upload sample preparation movies here | Please upload sample preparation movies here | ||
- | |||
- | === | + | = Microscope usage = |
+ | |||
+ | == Light Microscopy Documents == | ||
Please upload microscope usage protocols here | Please upload microscope usage protocols here | ||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
+ | '''Confocal microscopes''' | ||
+ | *[http://www.bioimaginguk.org/images/6/69/Leica_SP2_upright.pdf Leica SP2 Upright Quickstart Guide] (141 kB) | ||
+ | *[http://www.bioimaginguk.org/images/a/ac/Leica_sp5_upright.pdf Leica SP5 Upright Quickstart Guide] (233 kB) | ||
+ | *[http://www.bioimaginguk.org/images/c/c2/Leica_sp5res_inv.pdf Leica SP5 Inverted Quickstart Guide] (217 kB) | ||
+ | *[http://www.bioimaginguk.org/images/c/c2/Leica_sp5res_inv.pdf Leica SP5 with Resonant Scanner Quickstart Guide] (217 kB) | ||
+ | *[http://www.bioimaginguk.org/images/6/62/Zeiss_lsm510_inv.pdf Zeiss LSM 510 Quickstart Guide] (353 kB) | ||
- | + | '''Widefield microscopes''' | |
- | + | ||
- | + | ||
- | + | ||
- | == | + | *[http://www.bioimaginguk.org/images/5/5a/Zeiss_axiovert200m_simplepci.pdf Zeiss Axiovert 200M with Hamamatsu Volocity Software Quickstart Guide] (281 kB) |
+ | *[http://www.bioimaginguk.org/images/6/69/Zeiss_axiovert200m_volocity.pdf Zeiss Axiovert 200M with Simple PCI Software Quickstart Guide] (400 kB) | ||
+ | |||
+ | == Light Microscopy Movies == | ||
Please upload microscope usage movies here | Please upload microscope usage movies here | ||
+ | *[http://www.nikoninstruments.com/en_GB/Learn-Explore/Video-Tutorials Ti Live Cell System Setup] | ||
- | == Microscopy | + | == Electron Microscopy Documents == |
+ | Please upload microscope usage protocols here | ||
+ | |||
+ | '''3View (Serial Block Face SEM)''' | ||
+ | |||
+ | == Electron Microscopy Movies == | ||
+ | Please upload microscope usage movies here | ||
+ | |||
+ | = Microscope maintenance = | ||
- | == | + | == Light Microscopy Documents == |
Please upload microscope maintenance protocols here | Please upload microscope maintenance protocols here | ||
- | == | + | == Light Microscopy Movies == |
+ | |||
+ | *[http://www.youtube.com/watch?v=2Y0u6rg_fjM Home built PALM/STORM microscope] | ||
+ | *[http://www.youtube.com/watch?v=6tLd_J7uh_Q 3D PALM/STORM with astigmatism] | ||
+ | *[http://www.youtube.com/watch?v=nmwrSBkevE4 Custom laser control] | ||
+ | *[http://www.nikoninstruments.com/en_GB/Learn-Explore/Video-Tutorials Maintenance videos including Cleaning Microscope Optics & Bulb Replacement] | ||
+ | *[http://www.ibiology.org/ibioeducation/taking-courses/finding-dirt-in-your-optical-system.html Finding dirt in your optical system] | ||
+ | |||
+ | == Electron Microscopy Documents == | ||
+ | Please upload microscope maintenance protocols here | ||
+ | |||
+ | '''3View (Serial Block Face SEM)''' | ||
+ | |||
+ | == Electron Microscopy Movies == | ||
Please upload microscope maintenance movies here | Please upload microscope maintenance movies here | ||
- | + | = General microscopy = | |
- | == | + | == Light Microscopy Documents == |
+ | * [http://www.bioimaginguk.org/images/d/d4/Good_imaging_practice.pdf Good Imaging Practice Overview] (343 kB) | ||
+ | * [http://www.bioimaginguk.org/images/b/bb/Correlative_super_resolution_using_dSTORM_and_SIM.pdf Comparison of SIM and STORM results] (963 kB) | ||
+ | |||
+ | == Light Microscopy Movies == | ||
+ | Please upload general microscopy movies here | ||
+ | |||
+ | *[http://www.ibiology.org/ibioeducation/taking-courses/eyepieces.html Eyepieces] | ||
+ | *[http://www.ibiology.org/ibioeducation/taking-courses/objectives-and-eyepieces.html Objectives and eyepieces] | ||
+ | *[http://www.ibiology.org/ibioeducation/taking-courses/features-of-an-objective.html Features of an objective lens] | ||
+ | *[http://www.ibiology.org/ibioeducation/taking-courses/phase-polarization-and-dic-microscopy-lab.html Phase, Polarization & DIC Microscopy] | ||
+ | *[http://www.ibiology.org/ibioeducation/taking-courses/ibiology-microscopy-short-course/dark-field-phase-contrast-polarization-and-differential-interference-contrast-dic-microscopy.html Dark Field, Phase Contrast, Polarisation and DIC] | ||
+ | *[http://www.ibiology.org/ibioeducation/taking-courses/polarized-light-and-its-interaction-with-material.html Polarized Light and its Interaction with Material] | ||
+ | *[http://www.ibiology.org/ibioeducation/taking-courses/correcting-for-spherical-aberration-with-a-correction-collar.html Correcting for spherical aberration] | ||
+ | *[http://www.ibiology.org/ibioeducation/taking-courses/demystifying-microscopes-disassembling-a-nikon-ti-eclipse.html Demystifying microscopes - disassembling a Nikon Ti Eclipse] | ||
+ | |||
+ | *[http://www.ibiology.org/ibioeducation/taking-courses/ibiology-microscopy-short-course/lenses-and-image-formation.html Lenses and image formation] | ||
+ | *[http://www.ibiology.org/ibioeducation/taking-courses/ibiology-microscopy-short-course/what-can-you-learn-with-a-light-microscope-2.html What you can learn with a light microscope] | ||
+ | *[http://www.ibiology.org/ibioeducation/taking-courses/ibiology-microscopy-short-course/choosing-the-right-microscopy-technique.html Choosing the right microscopy technique] | ||
+ | *[http://www.ibiology.org/ibioeducation/taking-courses/summary-designing-a-fluorescence-microscopy-experiment.html Designing a fluorescence microscopy experiment] | ||
+ | *[http://www.ibiology.org/ibioeducation/taking-courses/introduction-to-fluorescence-microscopy.html Introduction to Fluorescence Microscopy] | ||
+ | *[http://www.ibiology.org/ibioeducation/taking-courses/deconvolution-microscopy.html Deconvolution] | ||
+ | *[http://www.ibiology.org/ibioeducation/taking-courses/ibiology-microscopy-short-course/resolution-in-microscopy.html Resolution in microscopy] | ||
+ | |||
+ | == Light Microscopy Advanced Techniques & Methods == | ||
+ | |||
+ | *[http://www.ibiology.org/ibioeducation/taking-courses/fourier-space.html Fourier Space (Image formation)] | ||
+ | *[http://www.ibiology.org/ibioeducation/taking-courses/high-throughput-microscopy.html Hight throughput microscopy] | ||
+ | *[http://www.ibiology.org/ibioeducation/taking-courses/deconvolution-microscopy.html Deconvolution] | ||
+ | *[http://www.ibiology.org/ibioeducation/taking-courses/optogenetics.html Optogenetics] | ||
+ | *[http://www.ibiology.org/ibioeducation/taking-courses/optical-sectioning-and-confocal-microscopy.html Optical sectioning and confocal microscopy] | ||
+ | *[http://www.ibiology.org/ibioeducation/taking-courses/total-internal-reflection-fluorescence-tirf-microscopy.html TIRF] | ||
+ | *[http://www.ibiology.org/ibioeducation/taking-courses/two-photon-microscopy.html Two photon microscopy] | ||
+ | |||
+ | '''F Words''' | ||
+ | *[http://www.ibiology.org/ibioeducation/taking-courses/forster-resonance-energy-transfer-fret-microscopy.html FRET] | ||
+ | *[http://www.jove.com/video/2149/imaging-protein-protein-interactions-in-vivo Imaging protein-protein interactions in-vivo (FRET)] | ||
+ | *[http://www.jove.com/video/2925/fluorescence-lifetime-imaging-of-molecular-rotors-in-living-cells Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells] | ||
+ | *[http://www.ibiology.org/ibioeducation/taking-courses/fluorescence-lifetime-imaging-microscopy.html FLIM] | ||
+ | *[http://www.jove.com/video/1876/fluorescent-labeling-cos-7-expressing-snap-tag-fusion-proteins-for Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging] | ||
+ | *[http://www.jove.com/video/3747/lateral-diffusion-exocytosis-membrane-proteins-cultured-neurons Lateral Diffusion and Exocytosis of Membrane Proteins in Cultured Neurons Assessed using Fluorescence Recovery and Fluorescence-loss Photobleaching] | ||
+ | *[http://www.ibiology.org/ibioeducation/taking-courses/ibiology-microscopy-short-course/measuring-dynamics-photobleaching-and-photoactivation-2.html FRAP and Photoactivation] | ||
+ | |||
+ | '''Super-Resolution Microscopy''' | ||
+ | *[http://www.ibiology.org/ibioeducation/taking-courses/ibiology-microscopy-short-course/super-resolution-microscopy.html Super-Resolution Microscopy] | ||
+ | *[http://www.ibiology.org/ibioeducation/taking-courses/super-resolution-overview-and-stimulated-emission-depletion-sted-microscopy.html Super-resolution microscopy: STED] | ||
+ | *[http://www.ibiology.org/ibioeducation/taking-courses/super-resolution-localization-microscopy.html Super-Resolution Microscopy: Localisation Microscopy] | ||
+ | *[http://www.ibiology.org/ibioeducation/taking-courses/super-resolution-structured-illumination-microscopy-sim.html Super-Resolution Microscopy: SIM] | ||
+ | *[http://www.jove.com/video/50579/test-samples-for-optimizing-storm-super-resolution-microscopy Test Samples for Optimizing STORM Super-Resolution Microscopy] | ||
+ | |||
+ | '''Light Sheet / SPIM''' | ||
+ | *[http://openspim.org/Welcome_to_the_OpenSPIM_Wiki OpenSPIM] | ||
+ | *[http://www.ibiology.org/ibioeducation/taking-courses/ibiology-microscopy-course/dual-view-inverted-selective-plane-illumination-dispim.html Dual view SPIM (DISPIM)] | ||
+ | *[http://www.ibiology.org/ibioeducation/taking-courses/light-sheet-sectioning.html Light Sheet/SPIM] | ||
+ | |||
+ | == Electron Microscopy Documents == | ||
Please upload general microscopy protocols here | Please upload general microscopy protocols here | ||
- | + | '''3View (Serial Block Face SEM)''' | |
- | == | + | == Electron Microscopy Movies == |
Please upload general microscopy movies here | Please upload general microscopy movies here | ||
- | + | = Software = | |
+ | |||
+ | == Documents == | ||
+ | |||
+ | *[http://icy.bioimageanalysis.org Icy: an open community platform for bioimage informatics] | ||
+ | *[http://code.google.com/p/quickpalm/ QuickPALM software for localisation microscopy] | ||
+ | *[http://wiki.laser.ceb.cam.ac.uk/wiki/index.php/Resources rainSTORM software for localisation microscopy] | ||
+ | *[http://www.bioimaginguk.org/index.php/File:RainSTORM_user_guide.pdf rainSTORM user guide] (1.31 MB) | ||
- | === | + | == Movies == |
- | + | ||
- | === | + | *[http://code.google.com/p/palm3d/ PALM3D] |
- | + | *[http://www.youtube.com/watch?v=_zgvo6GBdX8 Installation and Basics of ImageJ] | |
+ | *[http://www.youtube.com/watch?v=j113MSm6rXE RGB and grayscale montage in ImageJ] | ||
+ | *[http://www.youtube.com/watch?v=KoqpCsha-UU ImageJ Seminar] | ||
+ | *[http://www.youtube.com/watch?v=PqHFsmS1_JY Automated Cell Counting in ImageJ] |
Current revision as of 12:03, 31 March 2015
Contents |
Sample preparation
Light Microscopy Documents
- Localisation Microscopy (STORM/PALM) Coverglass Guide (745 kB)
- Localisation Microscopy (STORM/GSDIM) Immunolabelling Guide (532 kB)
Light Microscopy Movies
Please upload sample preparation movies here
Electron Microscopy Documents
Please upload sample preparation protocols here
3View (Serial Block Face SEM)
Electron Microscopy Movies
Please upload sample preparation movies here
Microscope usage
Light Microscopy Documents
Please upload microscope usage protocols here
Confocal microscopes
- Leica SP2 Upright Quickstart Guide (141 kB)
- Leica SP5 Upright Quickstart Guide (233 kB)
- Leica SP5 Inverted Quickstart Guide (217 kB)
- Leica SP5 with Resonant Scanner Quickstart Guide (217 kB)
- Zeiss LSM 510 Quickstart Guide (353 kB)
Widefield microscopes
- Zeiss Axiovert 200M with Hamamatsu Volocity Software Quickstart Guide (281 kB)
- Zeiss Axiovert 200M with Simple PCI Software Quickstart Guide (400 kB)
Light Microscopy Movies
Please upload microscope usage movies here
Electron Microscopy Documents
Please upload microscope usage protocols here
3View (Serial Block Face SEM)
Electron Microscopy Movies
Please upload microscope usage movies here
Microscope maintenance
Light Microscopy Documents
Please upload microscope maintenance protocols here
Light Microscopy Movies
- Home built PALM/STORM microscope
- 3D PALM/STORM with astigmatism
- Custom laser control
- Maintenance videos including Cleaning Microscope Optics & Bulb Replacement
- Finding dirt in your optical system
Electron Microscopy Documents
Please upload microscope maintenance protocols here
3View (Serial Block Face SEM)
Electron Microscopy Movies
Please upload microscope maintenance movies here
General microscopy
Light Microscopy Documents
- Good Imaging Practice Overview (343 kB)
- Comparison of SIM and STORM results (963 kB)
Light Microscopy Movies
Please upload general microscopy movies here
- Eyepieces
- Objectives and eyepieces
- Features of an objective lens
- Phase, Polarization & DIC Microscopy
- Dark Field, Phase Contrast, Polarisation and DIC
- Polarized Light and its Interaction with Material
- Correcting for spherical aberration
- Demystifying microscopes - disassembling a Nikon Ti Eclipse
- Lenses and image formation
- What you can learn with a light microscope
- Choosing the right microscopy technique
- Designing a fluorescence microscopy experiment
- Introduction to Fluorescence Microscopy
- Deconvolution
- Resolution in microscopy
Light Microscopy Advanced Techniques & Methods
- Fourier Space (Image formation)
- Hight throughput microscopy
- Deconvolution
- Optogenetics
- Optical sectioning and confocal microscopy
- TIRF
- Two photon microscopy
F Words
- FRET
- Imaging protein-protein interactions in-vivo (FRET)
- Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
- FLIM
- Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging
- Lateral Diffusion and Exocytosis of Membrane Proteins in Cultured Neurons Assessed using Fluorescence Recovery and Fluorescence-loss Photobleaching
- FRAP and Photoactivation
Super-Resolution Microscopy
- Super-Resolution Microscopy
- Super-resolution microscopy: STED
- Super-Resolution Microscopy: Localisation Microscopy
- Super-Resolution Microscopy: SIM
- Test Samples for Optimizing STORM Super-Resolution Microscopy
Light Sheet / SPIM
Electron Microscopy Documents
Please upload general microscopy protocols here
3View (Serial Block Face SEM)
Electron Microscopy Movies
Please upload general microscopy movies here
Software
Documents
- Icy: an open community platform for bioimage informatics
- QuickPALM software for localisation microscopy
- rainSTORM software for localisation microscopy
- rainSTORM user guide (1.31 MB)