Training & Documents
From BioImagingUKWiki
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*[http://www.ibiology.org/ibioeducation/taking-courses/ibiology-microscopy-short-course/resolution-in-microscopy.html Resolution in microscopy] | *[http://www.ibiology.org/ibioeducation/taking-courses/ibiology-microscopy-short-course/resolution-in-microscopy.html Resolution in microscopy] | ||
- | == | + | == Advanced Techniques & Methods (Open Access) == |
- | *[http://www. | + | |
- | *[http://www. | + | |
- | *[http://www. | + | *[http://www.ibiology.org/ibioeducation/taking-courses/light-sheet-sectioning.html Light Sheet/SPIM] |
+ | *[http://www.ibiology.org/ibioeducation/taking-courses/fourier-space.html Fourier Space (Image formation)] | ||
+ | *[http://www.ibiology.org/ibioeducation/taking-courses/high-throughput-microscopy.html] | ||
+ | *[http://www.ibiology.org/ibioeducation/taking-courses/deconvolution-microscopy.html] | ||
+ | *[http://www.ibiology.org/ibioeducation/taking-courses/optogenetics.html] | ||
+ | *[http://www.ibiology.org/ibioeducation/taking-courses/optical-sectioning-and-confocal-microscopy.html Optical sectioning and confocal microscopy] | ||
+ | |||
+ | '''F Words''' | ||
+ | *[http://www.ibiology.org/ibioeducation/taking-courses/forster-resonance-energy-transfer-fret-microscopy.html FRET] | ||
*[http://www.jove.com/video/2149/imaging-protein-protein-interactions-in-vivo Imaging protein-protein interactions in-vivo (FRET)] | *[http://www.jove.com/video/2149/imaging-protein-protein-interactions-in-vivo Imaging protein-protein interactions in-vivo (FRET)] | ||
+ | *[http://www.jove.com/video/2925/fluorescence-lifetime-imaging-of-molecular-rotors-in-living-cells Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells] | ||
+ | *[http://www.ibiology.org/ibioeducation/taking-courses/fluorescence-lifetime-imaging-microscopy.html FLIM] | ||
*[http://www.jove.com/video/1876/fluorescent-labeling-cos-7-expressing-snap-tag-fusion-proteins-for Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging] | *[http://www.jove.com/video/1876/fluorescent-labeling-cos-7-expressing-snap-tag-fusion-proteins-for Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging] | ||
+ | *[http://www.jove.com/video/3747/lateral-diffusion-exocytosis-membrane-proteins-cultured-neurons Lateral Diffusion and Exocytosis of Membrane Proteins in Cultured Neurons Assessed using Fluorescence Recovery and Fluorescence-loss Photobleaching] | ||
+ | *[http://www.ibiology.org/ibioeducation/taking-courses/ibiology-microscopy-short-course/measuring-dynamics-photobleaching-and-photoactivation-2.html FRAP and Photoactivation] | ||
+ | |||
+ | '''Super-Resolution Microscopy''' | ||
+ | *[http://www.jove.com/video/50579/test-samples-for-optimizing-storm-super-resolution-microscopy Test Samples for Optimizing STORM Super-Resolution Microscopy] | ||
+ | *[http://www.ibiology.org/ibioeducation/taking-courses/super-resolution-overview-and-stimulated-emission-depletion-sted-microscopy.html Super-resolution micrscopy:STED] | ||
= Software = | = Software = |
Revision as of 12:04, 22 December 2014
Contents |
Sample preparation
Documents
- Localisation Microscopy (STORM/PALM) Coverglass Guide (745 kB)
- Localisation Microscopy (STORM/GSDIM) Immunolabelling Guide (532 kB)
Movies
Please upload sample preparation movies here
Microscope usage
Documents
Please upload microscope usage protocols here
Confocal microscopes
- Leica SP2 Upright Quickstart Guide (141 kB)
- Leica SP5 Upright Quickstart Guide (233 kB)
- Leica SP5 Inverted Quickstart Guide (217 kB)
- Leica SP5 with Resonant Scanner Quickstart Guide (217 kB)
- Zeiss LSM 510 Quickstart Guide (353 kB)
Widefield microscopes
- Zeiss Axiovert 200M with Hamamatsu Volocity Software Quickstart Guide (281 kB)
- Zeiss Axiovert 200M with Simple PCI Software Quickstart Guide (400 kB)
Movies
Please upload microscope usage movies here
Microscopy maintenance
Documents
Please upload microscope maintenance protocols here
Movies
- Home built PALM/STORM microscope
- 3D PALM/STORM with astigmatism
- Custom laser control
- Maintenance videos including Cleaning Microscope Optics & Bulb Replacement
- Finding dirt in your optical system
General microscopy
Documents
- Good Imaging Practice Overview (343 kB)
- Comparison of SIM and STORM results (963 kB)
Movies
Please upload general microscopy movies here
- Eyepieces
- Objectives and eyepieces
- Features of an objective lens
- Phase, Polarization & DIC Microscopy
- Dark Field, Phase Contrast, Polarisation and DIC
- Polarized Light and its Interaction with Material
- Correcting for spherical aberration
- Demystifying microscopes - disassembling a Nikon Ti Eclipse
Advanced Techniques & Methods (Open Access)
- Light Sheet/SPIM
- Fourier Space (Image formation)
- [1]
- [2]
- [3]
- Optical sectioning and confocal microscopy
F Words
- FRET
- Imaging protein-protein interactions in-vivo (FRET)
- Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
- FLIM
- Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging
- Lateral Diffusion and Exocytosis of Membrane Proteins in Cultured Neurons Assessed using Fluorescence Recovery and Fluorescence-loss Photobleaching
- FRAP and Photoactivation
Super-Resolution Microscopy
Software
Documents
- Icy: an open community platform for bioimage informatics
- QuickPALM software for localisation microscopy
- rainSTORM software for localisation microscopy
- rainSTORM user guide (1.31 MB)