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Revision as of 22:12, 26 June 2013 (view source) Dmetcalf (Talk | contribs) (→Microscopy maintenance) ← Older edit		Revision as of 22:12, 26 June 2013 (view source) Dmetcalf (Talk | contribs) (→General microscopy) Newer edit →
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	*[http://www.youtube.com/watch?v=nmwrSBkevE4 Custom laser control]		*[http://www.youtube.com/watch?v=nmwrSBkevE4 Custom laser control]
-	== General microscopy ==	+	= General microscopy =
	=== Documents ===		=== Documents ===

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Revision as of 22:12, 26 June 2013

Contents 1 Sample preparation 1.1 Documents 1.2 Movies 2 Microscope usage 2.1 Documents 2.1.1 Confocal microscopes 2.1.2 Widefield microscopes 2.1.3 Movies 3 Microscopy maintenance 3.1 Documents 3.2 Movies 4 General microscopy 4.1 Documents 4.2 Movies 4.3 Journal of Visualized Experiments Microscopy Videos 4.4 Software 4.4.1 Documents 4.4.2 Movies

Sample preparation

Documents

• Localisation Microscopy (STORM/PALM) Coverglass Guide (745 kB)
• Localisation Microscopy (STORM/GSDIM) Immunolabelling Guide (532 kB)

Movies

Please upload sample preparation movies here

Microscope usage

Documents

Please upload microscope usage protocols here

Confocal microscopes

• Leica SP2 Upright Quickstart Guide (141 kB)
• Leica SP5 Upright Quickstart Guide (233 kB)
• Leica SP5 Inverted Quickstart Guide (217 kB)
• Leica SP5 with Resonant Scanner Quickstart Guide (217 kB)
• Zeiss LSM 510 Quickstart Guide (353 kB)

Widefield microscopes

• Zeiss Axiovert 200M with Hamamatsu Volocity Software Quickstart Guide (281 kB)
• Zeiss Axiovert 200M with Simple PCI Software Quickstart Guide (400 kB)

Movies

Please upload microscope usage movies here

Microscopy maintenance

Documents

Please upload microscope maintenance protocols here

Movies

• Home built PALM/STORM microscope
• 3D PALM/STORM with astigmatism
• Custom laser control

General microscopy

Documents

• Good Imaging Practice Overview (343 kB)
• Comparison of SIM and STORM results (963 kB)

Movies

Please upload general microscopy movies here

Journal of Visualized Experiments Microscopy Videos

• Lateral Diffusion and Exocytosis of Membrane Proteins in Cultured Neurons Assessed using Fluorescence Recovery and Fluorescence-loss Photobleaching
• Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Software

Documents

• Icy: an open community platform for bioimage informatics
• rainSTORM software for localisation microscopy
• QuickPALM software for localisation microscopy

Movies

• PALM3D
• Installation and Basics of ImageJ
• RGB and grayscale montage in ImageJ
• ImageJ Seminar
• Automated Cell Counting in ImageJ