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Revision as of 07:12, 22 December 2014 (view source) Dmetcalf (Talk | contribs) (→Movies) ← Older edit		Revision as of 07:28, 22 December 2014 (view source) Dmetcalf (Talk | contribs) (→Movies) Newer edit →
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	== Movies ==		== Movies ==
	Please upload general microscopy movies here		Please upload general microscopy movies here
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		+	*[http://www.ibiology.org/ibioeducation/taking-courses/features-of-an-objective.html Features of an objective lens]
		+	*[http://www.ibiology.org/ibioeducation/taking-courses/deconvolution-microscopy.html Deconvolution]
		+	*[http://www.ibiology.org/ibioeducation/taking-courses/ibiology-microscopy-short-course/resolution-in-microscopy.html Resolution in microscopy]
	== Journal of Visualized Experiments Microscopy Videos (Open Access) ==		== Journal of Visualized Experiments Microscopy Videos (Open Access) ==

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Revision as of 07:28, 22 December 2014

Contents 1 Sample preparation 1.1 Documents 1.2 Movies 2 Microscope usage 2.1 Documents 2.2 Movies 3 Microscopy maintenance 3.1 Documents 3.2 Movies 4 General microscopy 4.1 Documents 4.2 Movies 4.3 Journal of Visualized Experiments Microscopy Videos (Open Access) 5 Software 5.1 Documents 5.2 Movies

Sample preparation

Documents

• Localisation Microscopy (STORM/PALM) Coverglass Guide (745 kB)
• Localisation Microscopy (STORM/GSDIM) Immunolabelling Guide (532 kB)

Movies

Please upload sample preparation movies here

Microscope usage

Documents

Please upload microscope usage protocols here

Confocal microscopes

• Leica SP2 Upright Quickstart Guide (141 kB)
• Leica SP5 Upright Quickstart Guide (233 kB)
• Leica SP5 Inverted Quickstart Guide (217 kB)
• Leica SP5 with Resonant Scanner Quickstart Guide (217 kB)
• Zeiss LSM 510 Quickstart Guide (353 kB)

Widefield microscopes

• Zeiss Axiovert 200M with Hamamatsu Volocity Software Quickstart Guide (281 kB)
• Zeiss Axiovert 200M with Simple PCI Software Quickstart Guide (400 kB)

Movies

Please upload microscope usage movies here

Microscopy maintenance

Documents

Please upload microscope maintenance protocols here

Movies

• Home built PALM/STORM microscope
• 3D PALM/STORM with astigmatism
• Custom laser control
• Maintenance videos including Cleaning Microscope Optics & Bulb Replacement

General microscopy

Documents

• Good Imaging Practice Overview (343 kB)
• Comparison of SIM and STORM results (963 kB)

Movies

Please upload general microscopy movies here

• Features of an objective lens
• Deconvolution
• Resolution in microscopy

Journal of Visualized Experiments Microscopy Videos (Open Access)

• Test Samples for Optimizing STORM Super-Resolution Microscopy
• Lateral Diffusion and Exocytosis of Membrane Proteins in Cultured Neurons Assessed using Fluorescence Recovery and Fluorescence-loss Photobleaching
• Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
• Imaging protein-protein interactions in-vivo (FRET)
• Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging

Software

Documents

• Icy: an open community platform for bioimage informatics
• QuickPALM software for localisation microscopy
• rainSTORM software for localisation microscopy
• rainSTORM user guide (1.31 MB)

Movies

• PALM3D
• Installation and Basics of ImageJ
• RGB and grayscale montage in ImageJ
• ImageJ Seminar
• Automated Cell Counting in ImageJ