Training & Documents
From BioImagingUKWiki
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== Electron Microscopy Documents == | == Electron Microscopy Documents == | ||
| - | + | Please upload sample preparation protocols here | |
== Electron Microscopy Movies == | == Electron Microscopy Movies == | ||
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= Microscope usage = | = Microscope usage = | ||
| - | == Documents == | + | == Light Microscopy Documents == |
Please upload microscope usage protocols here | Please upload microscope usage protocols here | ||
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*[http://www.bioimaginguk.org/images/6/69/Zeiss_axiovert200m_volocity.pdf Zeiss Axiovert 200M with Simple PCI Software Quickstart Guide] (400 kB) | *[http://www.bioimaginguk.org/images/6/69/Zeiss_axiovert200m_volocity.pdf Zeiss Axiovert 200M with Simple PCI Software Quickstart Guide] (400 kB) | ||
| - | == Movies == | + | == Light Microscopy Movies == |
Please upload microscope usage movies here | Please upload microscope usage movies here | ||
*[http://www.nikoninstruments.com/en_GB/Learn-Explore/Video-Tutorials Ti Live Cell System Setup] | *[http://www.nikoninstruments.com/en_GB/Learn-Explore/Video-Tutorials Ti Live Cell System Setup] | ||
| + | |||
| + | == Electron Microscopy Documents == | ||
| + | Please upload microscope usage protocols here | ||
| + | |||
| + | == Electron Microscopy Movies == | ||
| + | Please upload microscope usage movies here | ||
= Microscopy maintenance = | = Microscopy maintenance = | ||
| - | == Documents == | + | == Light Microscopy Documents == |
Please upload microscope maintenance protocols here | Please upload microscope maintenance protocols here | ||
| - | == Movies == | + | == Light Microscopy Movies == |
*[http://www.youtube.com/watch?v=2Y0u6rg_fjM Home built PALM/STORM microscope] | *[http://www.youtube.com/watch?v=2Y0u6rg_fjM Home built PALM/STORM microscope] | ||
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*[http://www.nikoninstruments.com/en_GB/Learn-Explore/Video-Tutorials Maintenance videos including Cleaning Microscope Optics & Bulb Replacement] | *[http://www.nikoninstruments.com/en_GB/Learn-Explore/Video-Tutorials Maintenance videos including Cleaning Microscope Optics & Bulb Replacement] | ||
*[http://www.ibiology.org/ibioeducation/taking-courses/finding-dirt-in-your-optical-system.html Finding dirt in your optical system] | *[http://www.ibiology.org/ibioeducation/taking-courses/finding-dirt-in-your-optical-system.html Finding dirt in your optical system] | ||
| + | |||
| + | == Electron Microscopy Documents == | ||
| + | Please upload microscope maintenance protocols here | ||
| + | |||
| + | == Electron Microscopy Movies == | ||
| + | Please upload microscope maintenance movies here | ||
= General microscopy = | = General microscopy = | ||
| - | == Documents == | + | == Light Microscopy Documents == |
* [http://www.bioimaginguk.org/images/d/d4/Good_imaging_practice.pdf Good Imaging Practice Overview] (343 kB) | * [http://www.bioimaginguk.org/images/d/d4/Good_imaging_practice.pdf Good Imaging Practice Overview] (343 kB) | ||
* [http://www.bioimaginguk.org/images/b/bb/Correlative_super_resolution_using_dSTORM_and_SIM.pdf Comparison of SIM and STORM results] (963 kB) | * [http://www.bioimaginguk.org/images/b/bb/Correlative_super_resolution_using_dSTORM_and_SIM.pdf Comparison of SIM and STORM results] (963 kB) | ||
| - | == Movies == | + | == Light Microscopy Movies == |
Please upload general microscopy movies here | Please upload general microscopy movies here | ||
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*[http://www.ibiology.org/ibioeducation/taking-courses/ibiology-microscopy-short-course/resolution-in-microscopy.html Resolution in microscopy] | *[http://www.ibiology.org/ibioeducation/taking-courses/ibiology-microscopy-short-course/resolution-in-microscopy.html Resolution in microscopy] | ||
| - | == Advanced Techniques & Methods == | + | == Light Microscopy Advanced Techniques & Methods == |
*[http://www.ibiology.org/ibioeducation/taking-courses/fourier-space.html Fourier Space (Image formation)] | *[http://www.ibiology.org/ibioeducation/taking-courses/fourier-space.html Fourier Space (Image formation)] | ||
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*[http://www.ibiology.org/ibioeducation/taking-courses/ibiology-microscopy-course/dual-view-inverted-selective-plane-illumination-dispim.html Dual view SPIM (DISPIM)] | *[http://www.ibiology.org/ibioeducation/taking-courses/ibiology-microscopy-course/dual-view-inverted-selective-plane-illumination-dispim.html Dual view SPIM (DISPIM)] | ||
*[http://www.ibiology.org/ibioeducation/taking-courses/light-sheet-sectioning.html Light Sheet/SPIM] | *[http://www.ibiology.org/ibioeducation/taking-courses/light-sheet-sectioning.html Light Sheet/SPIM] | ||
| + | |||
| + | == Electron Microscopy Documents == | ||
| + | Please upload general microscopy protocols here | ||
| + | |||
| + | == Electron Microscopy Movies == | ||
| + | Please upload general microscopy movies here | ||
= Software = | = Software = | ||
Revision as of 20:00, 8 January 2015
Contents |
Sample preparation
Light Microscopy Documents
- Localisation Microscopy (STORM/PALM) Coverglass Guide (745 kB)
- Localisation Microscopy (STORM/GSDIM) Immunolabelling Guide (532 kB)
Light Microscopy Movies
Please upload sample preparation movies here
Electron Microscopy Documents
Please upload sample preparation protocols here
Electron Microscopy Movies
Please upload sample preparation movies here
Microscope usage
Light Microscopy Documents
Please upload microscope usage protocols here
Confocal microscopes
- Leica SP2 Upright Quickstart Guide (141 kB)
- Leica SP5 Upright Quickstart Guide (233 kB)
- Leica SP5 Inverted Quickstart Guide (217 kB)
- Leica SP5 with Resonant Scanner Quickstart Guide (217 kB)
- Zeiss LSM 510 Quickstart Guide (353 kB)
Widefield microscopes
- Zeiss Axiovert 200M with Hamamatsu Volocity Software Quickstart Guide (281 kB)
- Zeiss Axiovert 200M with Simple PCI Software Quickstart Guide (400 kB)
Light Microscopy Movies
Please upload microscope usage movies here
Electron Microscopy Documents
Please upload microscope usage protocols here
Electron Microscopy Movies
Please upload microscope usage movies here
Microscopy maintenance
Light Microscopy Documents
Please upload microscope maintenance protocols here
Light Microscopy Movies
- Home built PALM/STORM microscope
- 3D PALM/STORM with astigmatism
- Custom laser control
- Maintenance videos including Cleaning Microscope Optics & Bulb Replacement
- Finding dirt in your optical system
Electron Microscopy Documents
Please upload microscope maintenance protocols here
Electron Microscopy Movies
Please upload microscope maintenance movies here
General microscopy
Light Microscopy Documents
- Good Imaging Practice Overview (343 kB)
- Comparison of SIM and STORM results (963 kB)
Light Microscopy Movies
Please upload general microscopy movies here
- Eyepieces
- Objectives and eyepieces
- Features of an objective lens
- Phase, Polarization & DIC Microscopy
- Dark Field, Phase Contrast, Polarisation and DIC
- Polarized Light and its Interaction with Material
- Correcting for spherical aberration
- Demystifying microscopes - disassembling a Nikon Ti Eclipse
- Lenses and image formation
- What you can learn with a light microscope
- Choosing the right microscopy technique
- Designing a fluorescence microscopy experiment
- Introduction to Fluorescence Microscopy
- Deconvolution
- Resolution in microscopy
Light Microscopy Advanced Techniques & Methods
- Fourier Space (Image formation)
- Hight throughput microscopy
- Deconvolution
- Optogenetics
- Optical sectioning and confocal microscopy
- TIRF
- Two photon microscopy
F Words
- FRET
- Imaging protein-protein interactions in-vivo (FRET)
- Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
- FLIM
- Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging
- Lateral Diffusion and Exocytosis of Membrane Proteins in Cultured Neurons Assessed using Fluorescence Recovery and Fluorescence-loss Photobleaching
- FRAP and Photoactivation
Super-Resolution Microscopy
- Super-Resolution Microscopy
- Super-resolution microscopy: STED
- Super-Resolution Microscopy: Localisation Microscopy
- Super-Resolution Microscopy: SIM
- Test Samples for Optimizing STORM Super-Resolution Microscopy
Light Sheet / SPIM
Electron Microscopy Documents
Please upload general microscopy protocols here
Electron Microscopy Movies
Please upload general microscopy movies here
Software
Documents
- Icy: an open community platform for bioimage informatics
- QuickPALM software for localisation microscopy
- rainSTORM software for localisation microscopy
- rainSTORM user guide (1.31 MB)
