Training & Documents
From BioImagingUKWiki
(Difference between revisions)
(→Movies) |
(→Advanced Techniques & Methods) |
||
| Line 99: | Line 99: | ||
'''Light Sheet / SPIM''' | '''Light Sheet / SPIM''' | ||
| + | *[http://openspim.org/Welcome_to_the_OpenSPIM_Wiki] | ||
*[http://www.ibiology.org/ibioeducation/taking-courses/ibiology-microscopy-course/dual-view-inverted-selective-plane-illumination-dispim.html Dual view SPIM (DISPIM)] | *[http://www.ibiology.org/ibioeducation/taking-courses/ibiology-microscopy-course/dual-view-inverted-selective-plane-illumination-dispim.html Dual view SPIM (DISPIM)] | ||
*[http://www.ibiology.org/ibioeducation/taking-courses/light-sheet-sectioning.html Light Sheet/SPIM] | *[http://www.ibiology.org/ibioeducation/taking-courses/light-sheet-sectioning.html Light Sheet/SPIM] | ||
Revision as of 12:25, 22 December 2014
Contents |
Sample preparation
Documents
- Localisation Microscopy (STORM/PALM) Coverglass Guide (745 kB)
- Localisation Microscopy (STORM/GSDIM) Immunolabelling Guide (532 kB)
Movies
Please upload sample preparation movies here
Microscope usage
Documents
Please upload microscope usage protocols here
Confocal microscopes
- Leica SP2 Upright Quickstart Guide (141 kB)
- Leica SP5 Upright Quickstart Guide (233 kB)
- Leica SP5 Inverted Quickstart Guide (217 kB)
- Leica SP5 with Resonant Scanner Quickstart Guide (217 kB)
- Zeiss LSM 510 Quickstart Guide (353 kB)
Widefield microscopes
- Zeiss Axiovert 200M with Hamamatsu Volocity Software Quickstart Guide (281 kB)
- Zeiss Axiovert 200M with Simple PCI Software Quickstart Guide (400 kB)
Movies
Please upload microscope usage movies here
Microscopy maintenance
Documents
Please upload microscope maintenance protocols here
Movies
- Home built PALM/STORM microscope
- 3D PALM/STORM with astigmatism
- Custom laser control
- Maintenance videos including Cleaning Microscope Optics & Bulb Replacement
- Finding dirt in your optical system
General microscopy
Documents
- Good Imaging Practice Overview (343 kB)
- Comparison of SIM and STORM results (963 kB)
Movies
Please upload general microscopy movies here
- Eyepieces
- Objectives and eyepieces
- Features of an objective lens
- Phase, Polarization & DIC Microscopy
- Dark Field, Phase Contrast, Polarisation and DIC
- Polarized Light and its Interaction with Material
- Correcting for spherical aberration
- Demystifying microscopes - disassembling a Nikon Ti Eclipse
- Lenses and image formation
- What you can learn with a light microscope
- Choosing the right microscopy technique
- Designing a fluorescence microscopy experiment
- Introduction to Fluorescence Microscopy
- Deconvolution
- Resolution in microscopy
Advanced Techniques & Methods
- Fourier Space (Image formation)
- Hight throughput microscopy
- Deconvolution
- Optogenetics
- Optical sectioning and confocal microscopy
- TIRF
- Two photon microscopy
F Words
- FRET
- Imaging protein-protein interactions in-vivo (FRET)
- Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
- FLIM
- Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging
- Lateral Diffusion and Exocytosis of Membrane Proteins in Cultured Neurons Assessed using Fluorescence Recovery and Fluorescence-loss Photobleaching
- FRAP and Photoactivation
Super-Resolution Microscopy
- Super-Resolution Microscopy
- Super-resolution microscopy: STED
- Super-Resolution Microscopy: Localisation Microscopy
- Super-Resolution Microscopy: SIM
- Test Samples for Optimizing STORM Super-Resolution Microscopy
Light Sheet / SPIM
Software
Documents
- Icy: an open community platform for bioimage informatics
- QuickPALM software for localisation microscopy
- rainSTORM software for localisation microscopy
- rainSTORM user guide (1.31 MB)
